Stability of Staphylococcus aureus Phage ISP after Freeze-Drying (Lyophilization)

Maia Merabishvili; Chris Vervaet; Jean Paul Pirnay; Daniel de Vos; Gilbert Verbeken; Jan Mast; Nino Chanishvili; Mario Vaneechoutte

doi:10.1371/journal.pone.0068797

Stability of Staphylococcus aureus Phage ISP after Freeze-Drying (Lyophilization)

Maia Merabishvili, Chris Vervaet, Jean Paul Pirnay, Daniel de Vos, Gilbert Verbeken, Jan Mast, Nino Chanishvili, Mario Vaneechoutte

Laboratory for Molecular and Cellular Technology

Résultats de recherche: Contribution à un journal › Article › Revue par des pairs

Résumé

Staphylococcus aureus phage ISP was lyophilized, using an Amsco-Finn Aqua GT4 freeze dryer, in the presence of six different stabilizers at different concentrations. Stability of the lyophilized phage at 4°C was monitored up to 37 months and compared to stability in Luria Bertani broth and physiological saline at 4°C. Sucrose and trehalose were shown to be the best stabilizing additives, causing a decrease of only 1 log immediately after the lyophilization procedure and showing high stability during a 27 month storage period.

langue originale	Anglais
Numéro d'article	e68797
journal	PLoS ONE
Volume	8
Numéro de publication	7
Les DOIs	https://doi.org/10.1371/journal.pone.0068797
état	Publié - 2 juil. 2013

Accès au document

10.1371/journal.pone.0068797

Autres fichiers et liens

Lien vers la publication dans Scopus

Contient cette citation

@article{6f6ae12c28bc46c298836ec8b79a6762,

title = "Stability of Staphylococcus aureus Phage ISP after Freeze-Drying (Lyophilization)",

abstract = "Staphylococcus aureus phage ISP was lyophilized, using an Amsco-Finn Aqua GT4 freeze dryer, in the presence of six different stabilizers at different concentrations. Stability of the lyophilized phage at 4°C was monitored up to 37 months and compared to stability in Luria Bertani broth and physiological saline at 4°C. Sucrose and trehalose were shown to be the best stabilizing additives, causing a decrease of only 1 log immediately after the lyophilization procedure and showing high stability during a 27 month storage period.",

author = "Maia Merabishvili and Chris Vervaet and Pirnay, {Jean Paul} and {de Vos}, Daniel and Gilbert Verbeken and Jan Mast and Nino Chanishvili and Mario Vaneechoutte",

year = "2013",

month = jul,

day = "2",

doi = "10.1371/journal.pone.0068797",

language = "English",

volume = "8",

journal = "PLoS ONE",

issn = "1932-6203",

number = "7",

}

TY - JOUR

T1 - Stability of Staphylococcus aureus Phage ISP after Freeze-Drying (Lyophilization)

AU - Merabishvili, Maia

AU - Vervaet, Chris

AU - Pirnay, Jean Paul

AU - de Vos, Daniel

AU - Verbeken, Gilbert

AU - Mast, Jan

AU - Chanishvili, Nino

AU - Vaneechoutte, Mario

PY - 2013/7/2

Y1 - 2013/7/2

N2 - Staphylococcus aureus phage ISP was lyophilized, using an Amsco-Finn Aqua GT4 freeze dryer, in the presence of six different stabilizers at different concentrations. Stability of the lyophilized phage at 4°C was monitored up to 37 months and compared to stability in Luria Bertani broth and physiological saline at 4°C. Sucrose and trehalose were shown to be the best stabilizing additives, causing a decrease of only 1 log immediately after the lyophilization procedure and showing high stability during a 27 month storage period.

AB - Staphylococcus aureus phage ISP was lyophilized, using an Amsco-Finn Aqua GT4 freeze dryer, in the presence of six different stabilizers at different concentrations. Stability of the lyophilized phage at 4°C was monitored up to 37 months and compared to stability in Luria Bertani broth and physiological saline at 4°C. Sucrose and trehalose were shown to be the best stabilizing additives, causing a decrease of only 1 log immediately after the lyophilization procedure and showing high stability during a 27 month storage period.

UR - http://www.scopus.com/inward/record.url?scp=84879747506&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0068797

DO - 10.1371/journal.pone.0068797

M3 - Article

C2 - 23844241

AN - SCOPUS:84879747506

SN - 1932-6203

VL - 8

JO - PLoS ONE

JF - PLoS ONE

IS - 7

M1 - e68797

ER -

Stability of Staphylococcus aureus Phage ISP after Freeze-Drying (Lyophilization)

Résumé

Accès au document

Autres fichiers et liens

Empreinte digitale

Contient cette citation