Development and optimization of a PCR assay for detection of Dobrava and Puumala hantaviruses in Bosnia and Herzegovina

Lejla Smajlović; Jon Davoren; Paul Heyman; Christel Cochez; Cordula Haas; Caroline Maake; Mirsada Hukić

doi:10.1016/j.jviromet.2012.03.003

Development and optimization of a PCR assay for detection of Dobrava and Puumala hantaviruses in Bosnia and Herzegovina

Lejla Smajlović, Jon Davoren, Paul Heyman, Christel Cochez, Cordula Haas, Caroline Maake, Mirsada Hukić

Laboratory for Molecular and Cellular Technology

Résultats de recherche: Contribution à un journal › Article › Revue par des pairs

Résumé

Hantavirus-specific serology tests are the main diagnostic technique for detection of hantavirus infection in Bosnia and Herzegovina. In order to enhance hantavirus infections monitoring a sensitive PCR based assay was developed to detect Dobrava (DOBV) and Puumala (PUUV) hantaviruses. Nested primer sets were designed within three different regions of the viral RNA (S and M segment of DOBV and M segment of PUUV) based on highly similar regions from a number of different European hantavirus strains. Assay conditions were optimized using cell cultures infected with DOBV Slovenia, PUUV Sotkamo and PUUV CG 18-20. This sensitive and specific assay has proven to be useful for detection of both Puumala and Dobrava hantaviruses.

langue originale	Anglais
Pages (de - à)	37-42
Nombre de pages	6
journal	Journal of Virological Methods
Volume	182
Numéro de publication	1-2
Les DOIs	https://doi.org/10.1016/j.jviromet.2012.03.003
état	Publié - juin 2012

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10.1016/j.jviromet.2012.03.003

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title = "Development and optimization of a PCR assay for detection of Dobrava and Puumala hantaviruses in Bosnia and Herzegovina",

abstract = "Hantavirus-specific serology tests are the main diagnostic technique for detection of hantavirus infection in Bosnia and Herzegovina. In order to enhance hantavirus infections monitoring a sensitive PCR based assay was developed to detect Dobrava (DOBV) and Puumala (PUUV) hantaviruses. Nested primer sets were designed within three different regions of the viral RNA (S and M segment of DOBV and M segment of PUUV) based on highly similar regions from a number of different European hantavirus strains. Assay conditions were optimized using cell cultures infected with DOBV Slovenia, PUUV Sotkamo and PUUV CG 18-20. This sensitive and specific assay has proven to be useful for detection of both Puumala and Dobrava hantaviruses.",

keywords = "Dobrava, Hantavirus, PCR optimization, Primers, Puumala",

author = "Lejla Smajlovi{\'c} and Jon Davoren and Paul Heyman and Christel Cochez and Cordula Haas and Caroline Maake and Mirsada Huki{\'c}",

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AU - Davoren, Jon

AU - Heyman, Paul

AU - Cochez, Christel

AU - Haas, Cordula

AU - Maake, Caroline

AU - Hukić, Mirsada

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AB - Hantavirus-specific serology tests are the main diagnostic technique for detection of hantavirus infection in Bosnia and Herzegovina. In order to enhance hantavirus infections monitoring a sensitive PCR based assay was developed to detect Dobrava (DOBV) and Puumala (PUUV) hantaviruses. Nested primer sets were designed within three different regions of the viral RNA (S and M segment of DOBV and M segment of PUUV) based on highly similar regions from a number of different European hantavirus strains. Assay conditions were optimized using cell cultures infected with DOBV Slovenia, PUUV Sotkamo and PUUV CG 18-20. This sensitive and specific assay has proven to be useful for detection of both Puumala and Dobrava hantaviruses.

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KW - Hantavirus

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Development and optimization of a PCR assay for detection of Dobrava and Puumala hantaviruses in Bosnia and Herzegovina

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