Quantitation of pseudomonas aeruginosa in wound biopsy samples: From bacterial culture to rapid 'real-time' polymerase chain reaction

Jean Paul Pirnay; Daniel De Vos; Luc Duinslaeger; Pascal Reper; Christian Vandenvelde; Pierre Cornelis; Alain Vanderkelen

doi:10.1186/cc702

Quantitation of pseudomonas aeruginosa in wound biopsy samples: From bacterial culture to rapid 'real-time' polymerase chain reaction

Jean Paul Pirnay, Daniel De Vos, Luc Duinslaeger, Pascal Reper, Christian Vandenvelde, Pierre Cornelis, Alain Vanderkelen

Research output: Contribution to journal › Article › peer-review

Abstract

We developed a real-time detection (RTD) polymerase chain reaction (PCR) with rapid thermal cycling to detect and quantify Pseudomonas aeruginosa in wound biopsy samples. This method produced a linear quantitative detection range of 7 logs, with a lower detection limit of 103 colony-forming units (CFU)/g tissue or a few copies per reaction. The time from sample collection to result was less than 1 h. RTD-PCR has potential for rapid quantitative detection of pathogens in critical care patients, enabling early and individualized treatment.

Original language	English
Pages (from-to)	255-261
Number of pages	7
Journal	Critical Care
Volume	4
Issue number	4
DOIs	https://doi.org/10.1186/cc702
Publication status	Published - 2000

Keywords

Burn wound
Polymerase chain reaction
Pseudomonas aeruginosa
Quantitation
Sepsis

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10.1186/cc702

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abstract = "We developed a real-time detection (RTD) polymerase chain reaction (PCR) with rapid thermal cycling to detect and quantify Pseudomonas aeruginosa in wound biopsy samples. This method produced a linear quantitative detection range of 7 logs, with a lower detection limit of 103 colony-forming units (CFU)/g tissue or a few copies per reaction. The time from sample collection to result was less than 1 h. RTD-PCR has potential for rapid quantitative detection of pathogens in critical care patients, enabling early and individualized treatment.",

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AU - Pirnay, Jean Paul

AU - De Vos, Daniel

AU - Duinslaeger, Luc

AU - Reper, Pascal

AU - Vandenvelde, Christian

AU - Cornelis, Pierre

AU - Vanderkelen, Alain

PY - 2000

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AB - We developed a real-time detection (RTD) polymerase chain reaction (PCR) with rapid thermal cycling to detect and quantify Pseudomonas aeruginosa in wound biopsy samples. This method produced a linear quantitative detection range of 7 logs, with a lower detection limit of 103 colony-forming units (CFU)/g tissue or a few copies per reaction. The time from sample collection to result was less than 1 h. RTD-PCR has potential for rapid quantitative detection of pathogens in critical care patients, enabling early and individualized treatment.

KW - Burn wound

KW - Polymerase chain reaction

KW - Pseudomonas aeruginosa

KW - Quantitation

KW - Sepsis

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JO - Critical Care

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ER -

Quantitation of pseudomonas aeruginosa in wound biopsy samples: From bacterial culture to rapid 'real-time' polymerase chain reaction

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Keywords

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