TY - JOUR
T1 - In vivo cell kinetic measurements in human oesophageal cancer
T2 - What can be learned from multiple biopsies?
AU - Haustermans, K.
AU - Vanuytsel, L.
AU - Geboes, K.
AU - Lerut, T.
AU - Van Thillo, J.
AU - Leysen, J.
AU - Coosemans, W.
AU - van der Schueren, E.
N1 - Funding Information:
Acknowledgements-This study was supported by “Het Belgisch Werk tegen Kanker”. We would like to express our thanks to M. Ramaekers and G. Pottie for technical assistance, to A. J. Van der Kogel Ph.D., P. Spaas M. D. and W. Landuyt for useful comments.
PY - 1994
Y1 - 1994
N2 - The importance of intratumour variability of cell kinetics was studied in 60 patients with cancer of the oesophagus. Five biopsies per tumour were taken. The labelling index, S-phase duration and potential doubling time (Tpot were measured using flaw cytometry. The mean Tpot value was 5.56 ± 4.43 days (± 1S.D.) for adenocarcinomas and 4.40 ± 2.45 days (± 1S.D.) for squamous cell carcinomas. These values were statistically significantly different. Although intratumour variation in Tpot measurements occurred, the intertumour variability was more important (P < 0.00001). This feature permits classification of tumours into slow and fast proliferating groups, leaving an intermediate group of tumours that could not be unequivocally categorised. The relative distribution of tumours into these three categories depends on the intratumour and intertumour variability of Tpot and on the cut-off values used. Increasing the number of biopsies from one to five reduces the number of non-classifiable tumours.
AB - The importance of intratumour variability of cell kinetics was studied in 60 patients with cancer of the oesophagus. Five biopsies per tumour were taken. The labelling index, S-phase duration and potential doubling time (Tpot were measured using flaw cytometry. The mean Tpot value was 5.56 ± 4.43 days (± 1S.D.) for adenocarcinomas and 4.40 ± 2.45 days (± 1S.D.) for squamous cell carcinomas. These values were statistically significantly different. Although intratumour variation in Tpot measurements occurred, the intertumour variability was more important (P < 0.00001). This feature permits classification of tumours into slow and fast proliferating groups, leaving an intermediate group of tumours that could not be unequivocally categorised. The relative distribution of tumours into these three categories depends on the intratumour and intertumour variability of Tpot and on the cut-off values used. Increasing the number of biopsies from one to five reduces the number of non-classifiable tumours.
KW - , flow cytometry, oesophageal neoplasms
KW - cell kinetics
UR - http://www.scopus.com/inward/record.url?scp=0027996276&partnerID=8YFLogxK
U2 - 10.1016/0959-8049(94)00252-Z
DO - 10.1016/0959-8049(94)00252-Z
M3 - Article
C2 - 7880607
AN - SCOPUS:0027996276
SN - 0959-8049
VL - 30
SP - 1787
EP - 1791
JO - European Journal of Cancer
JF - European Journal of Cancer
IS - 12
ER -