Guidelines to compose an ideal bacteriophage cocktail

Maia Merabishvili, Jean Paul Pirnay, Daniel De Vos

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Correctly designed bacteriophage therapeutics are the cornerstone for a successful outcome of bacteriophage therapy. Here we overview strategies on how to choose bacteriophages and their bacterial hosts at different steps of a bacteriophage cocktail development in order to comply with all quality and safety requirements based on the already existing essentially empirical experience in bacteriophage therapy and current accomplishments in modern biomedical sciences. A modification of the classic Appelmans’ method (1922) to assess stability of bacteriophage activity in liquid media is presented in order to improve the overall performance of therapeutic bacteriophages individually and collectively in the cocktail.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages99-110
Number of pages12
DOIs
Publication statusPublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1693
ISSN (Print)1064-3745

Keywords

  • Adaptation of bacteriophages
  • Bacteria and bacteriophage of choice
  • Bacteriophage compatibility
  • Bacteriophage therapeutics
  • Host range
  • Virulent bacteriophages

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