Development and optimization of a PCR assay for detection of Dobrava and Puumala hantaviruses in Bosnia and Herzegovina

Lejla Smajlović; Jon Davoren; Paul Heyman; Christel Cochez; Cordula Haas; Caroline Maake; Mirsada Hukić

doi:10.1016/j.jviromet.2012.03.003

Development and optimization of a PCR assay for detection of Dobrava and Puumala hantaviruses in Bosnia and Herzegovina

Lejla Smajlović
, Jon Davoren
, Paul Heyman
, Christel Cochez
, Cordula Haas
, Caroline Maake
, Mirsada Hukić

Laboratory for Molecular and Cellular Technology

International Commission on Missing Persons
The Bode Technology Group, Inc.
Military Hospital Queen Astrid
University of Zurich
Clinical Center University of Sarajevo

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Hantavirus-specific serology tests are the main diagnostic technique for detection of hantavirus infection in Bosnia and Herzegovina. In order to enhance hantavirus infections monitoring a sensitive PCR based assay was developed to detect Dobrava (DOBV) and Puumala (PUUV) hantaviruses. Nested primer sets were designed within three different regions of the viral RNA (S and M segment of DOBV and M segment of PUUV) based on highly similar regions from a number of different European hantavirus strains. Assay conditions were optimized using cell cultures infected with DOBV Slovenia, PUUV Sotkamo and PUUV CG 18-20. This sensitive and specific assay has proven to be useful for detection of both Puumala and Dobrava hantaviruses.

Original language	English
Pages (from-to)	37-42
Number of pages	6
Journal	Journal of Virological Methods
Volume	182
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jviromet.2012.03.003
Publication status	Published - Jun 2012

Keywords

Dobrava
Hantavirus
PCR optimization
Primers
Puumala

Access to Document

10.1016/j.jviromet.2012.03.003

Cite this

@article{a1c3c41b20cc4b9386fda6bce4577fb2,

title = "Development and optimization of a PCR assay for detection of Dobrava and Puumala hantaviruses in Bosnia and Herzegovina",

abstract = "Hantavirus-specific serology tests are the main diagnostic technique for detection of hantavirus infection in Bosnia and Herzegovina. In order to enhance hantavirus infections monitoring a sensitive PCR based assay was developed to detect Dobrava (DOBV) and Puumala (PUUV) hantaviruses. Nested primer sets were designed within three different regions of the viral RNA (S and M segment of DOBV and M segment of PUUV) based on highly similar regions from a number of different European hantavirus strains. Assay conditions were optimized using cell cultures infected with DOBV Slovenia, PUUV Sotkamo and PUUV CG 18-20. This sensitive and specific assay has proven to be useful for detection of both Puumala and Dobrava hantaviruses.",

keywords = "Dobrava, Hantavirus, PCR optimization, Primers, Puumala",

author = "Lejla Smajlovi{\'c} and Jon Davoren and Paul Heyman and Christel Cochez and Cordula Haas and Caroline Maake and Mirsada Huki{\'c}",

year = "2012",

month = jun,

doi = "10.1016/j.jviromet.2012.03.003",

language = "English",

volume = "182",

pages = "37--42",

journal = "Journal of Virological Methods",

issn = "0166-0934",

number = "1-2",

}

TY - JOUR

T1 - Development and optimization of a PCR assay for detection of Dobrava and Puumala hantaviruses in Bosnia and Herzegovina

AU - Smajlović, Lejla

AU - Davoren, Jon

AU - Heyman, Paul

AU - Cochez, Christel

AU - Haas, Cordula

AU - Maake, Caroline

AU - Hukić, Mirsada

PY - 2012/6

Y1 - 2012/6

N2 - Hantavirus-specific serology tests are the main diagnostic technique for detection of hantavirus infection in Bosnia and Herzegovina. In order to enhance hantavirus infections monitoring a sensitive PCR based assay was developed to detect Dobrava (DOBV) and Puumala (PUUV) hantaviruses. Nested primer sets were designed within three different regions of the viral RNA (S and M segment of DOBV and M segment of PUUV) based on highly similar regions from a number of different European hantavirus strains. Assay conditions were optimized using cell cultures infected with DOBV Slovenia, PUUV Sotkamo and PUUV CG 18-20. This sensitive and specific assay has proven to be useful for detection of both Puumala and Dobrava hantaviruses.

AB - Hantavirus-specific serology tests are the main diagnostic technique for detection of hantavirus infection in Bosnia and Herzegovina. In order to enhance hantavirus infections monitoring a sensitive PCR based assay was developed to detect Dobrava (DOBV) and Puumala (PUUV) hantaviruses. Nested primer sets were designed within three different regions of the viral RNA (S and M segment of DOBV and M segment of PUUV) based on highly similar regions from a number of different European hantavirus strains. Assay conditions were optimized using cell cultures infected with DOBV Slovenia, PUUV Sotkamo and PUUV CG 18-20. This sensitive and specific assay has proven to be useful for detection of both Puumala and Dobrava hantaviruses.

KW - Dobrava

KW - Hantavirus

KW - PCR optimization

KW - Primers

KW - Puumala

UR - https://www.scopus.com/pages/publications/84860003366

U2 - 10.1016/j.jviromet.2012.03.003

DO - 10.1016/j.jviromet.2012.03.003

M3 - Article

C2 - 22433513

AN - SCOPUS:84860003366

SN - 0166-0934

VL - 182

SP - 37

EP - 42

JO - Journal of Virological Methods

JF - Journal of Virological Methods

IS - 1-2

ER -

Development and optimization of a PCR assay for detection of Dobrava and Puumala hantaviruses in Bosnia and Herzegovina

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this