Development and optimization of a PCR assay for detection of Dobrava and Puumala hantaviruses in Bosnia and Herzegovina

Lejla Smajlović; Jon Davoren; Paul Heyman; Christel Cochez; Cordula Haas; Caroline Maake; Mirsada Hukić

doi:10.1016/j.jviromet.2012.03.003

Development and optimization of a PCR assay for detection of Dobrava and Puumala hantaviruses in Bosnia and Herzegovina

Lejla Smajlović
, Jon Davoren
, Paul Heyman
, Christel Cochez
, Cordula Haas
, Caroline Maake
, Mirsada Hukić

Laboratory for Molecular and Cellular Technology

International Commission on Missing Persons
The Bode Technology Group, Inc.
Military Hospital Queen Astrid
University of Zurich
Clinical Center University of Sarajevo

Publikation: Beitrag in Fachzeitschrift › Artikel › Begutachtung

1 Zitat (Scopus)

Abstract

Hantavirus-specific serology tests are the main diagnostic technique for detection of hantavirus infection in Bosnia and Herzegovina. In order to enhance hantavirus infections monitoring a sensitive PCR based assay was developed to detect Dobrava (DOBV) and Puumala (PUUV) hantaviruses. Nested primer sets were designed within three different regions of the viral RNA (S and M segment of DOBV and M segment of PUUV) based on highly similar regions from a number of different European hantavirus strains. Assay conditions were optimized using cell cultures infected with DOBV Slovenia, PUUV Sotkamo and PUUV CG 18-20. This sensitive and specific assay has proven to be useful for detection of both Puumala and Dobrava hantaviruses.

Originalsprache	Englisch
Seiten (von - bis)	37-42
Seitenumfang	6
Fachzeitschrift	Journal of Virological Methods
Jahrgang	182
Ausgabenummer	1-2
DOIs	https://doi.org/10.1016/j.jviromet.2012.03.003
Publikationsstatus	Veröffentlicht - Juni 2012

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10.1016/j.jviromet.2012.03.003

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abstract = "Hantavirus-specific serology tests are the main diagnostic technique for detection of hantavirus infection in Bosnia and Herzegovina. In order to enhance hantavirus infections monitoring a sensitive PCR based assay was developed to detect Dobrava (DOBV) and Puumala (PUUV) hantaviruses. Nested primer sets were designed within three different regions of the viral RNA (S and M segment of DOBV and M segment of PUUV) based on highly similar regions from a number of different European hantavirus strains. Assay conditions were optimized using cell cultures infected with DOBV Slovenia, PUUV Sotkamo and PUUV CG 18-20. This sensitive and specific assay has proven to be useful for detection of both Puumala and Dobrava hantaviruses.",

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author = "Lejla Smajlovi{\'c} and Jon Davoren and Paul Heyman and Christel Cochez and Cordula Haas and Caroline Maake and Mirsada Huki{\'c}",

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AU - Davoren, Jon

AU - Heyman, Paul

AU - Cochez, Christel

AU - Haas, Cordula

AU - Maake, Caroline

AU - Hukić, Mirsada

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Development and optimization of a PCR assay for detection of Dobrava and Puumala hantaviruses in Bosnia and Herzegovina

Abstract

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