TY - JOUR
T1 - Characterization of newly isolated lytic bacteriophages active against Acinetobacter baumannii
AU - Merabishvili, Maia
AU - Vandenheuvel, Dieter
AU - Kropinski, Andrew M.
AU - Mast, Jan
AU - De Vos, Daniel
AU - Verbeken, Gilbert
AU - Noben, Jean Paul
AU - Lavigne, Rob
AU - Vaneechoutte, Mario
AU - Pirnay, Jean Paul
PY - 2014/8/11
Y1 - 2014/8/11
N2 - Based on genotyping and host range, two newly isolated lytic bacteriophages, myovirus vB-AbaM-Acibel004 and podovirus vB-AbaP-Acibel007, active against Acinetobacter baumannii clinical strains, were selected from a new phage library for further characterization. The complete genomes of the two phages were analyzed. Both phages are characterized by broad host range and essential features of potential therapeutic phages, such as short latent period (27 and 21 min, respectively), high burst size (125 and 145, respectively), stability of activity in liquid culture and low frequency of occurrence of phage-resistant mutant bacterial cells. Genomic analysis showed that while Acibel004 represents a novel bacteriophage with resemblance to some unclassified Pseudomonas aeruginosa phages, Acibel007 belongs to the well-characterized genus of the Phikmvlikevirus. The newly isolated phages can serve as potential candidates for phage cocktails to control A. baumannii infections.
AB - Based on genotyping and host range, two newly isolated lytic bacteriophages, myovirus vB-AbaM-Acibel004 and podovirus vB-AbaP-Acibel007, active against Acinetobacter baumannii clinical strains, were selected from a new phage library for further characterization. The complete genomes of the two phages were analyzed. Both phages are characterized by broad host range and essential features of potential therapeutic phages, such as short latent period (27 and 21 min, respectively), high burst size (125 and 145, respectively), stability of activity in liquid culture and low frequency of occurrence of phage-resistant mutant bacterial cells. Genomic analysis showed that while Acibel004 represents a novel bacteriophage with resemblance to some unclassified Pseudomonas aeruginosa phages, Acibel007 belongs to the well-characterized genus of the Phikmvlikevirus. The newly isolated phages can serve as potential candidates for phage cocktails to control A. baumannii infections.
UR - http://www.scopus.com/inward/record.url?scp=84905841643&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0104853
DO - 10.1371/journal.pone.0104853
M3 - Article
C2 - 25111143
AN - SCOPUS:84905841643
SN - 1932-6203
VL - 9
JO - PLoS ONE
JF - PLoS ONE
IS - 8
M1 - e104853
ER -